Associated thus FE provides the ability to correct pairing antikodona with a codon, but preclude the inclusion of the amino acid to the growing peptide. The initial recognition of the codon is used for PV signal to hydrolysis of GTP to GDP P, after which the FA is separated from the Ribosome with tRNA and protein synthesis continues. Thanks to FA there is a short delay between pairing codons with antikodonom and elongation of the peptide, that allows you to separate itself from tRNA the Ribosome.
«Wrong» molecule of tRNA forms a couple of codon-antikodon less hydrogen bonds, than the correct; It therefore weaker hold on ribosome and thus over a period of time is more likely to secede. ". Commenting on this, important for us, longest shutter speed, You can say, the emphasis it placed on the mutual recognition of tRNA and amino acids through the aminoacyl-tRNA-synthetases are studied. The mechanism is not clear. With regard to the accuracy of the recognition of codon antikodona, It is an illusion because of the "voblirovaniâ" of the third nucleotide, as discussed.
It Seems, the choice of dubletnyh-homonyms is realized by codons resonant wave and context (the associative, holographic) and the so-called "background processes" (see. below). So far, they were out of experiments and reasoning, but now this is obvious. Omonimičnost′ (ambiguity) the code can be overcome in exactly the same way, as in natural languages ,- by placing the homonym, as part of the, to integer, i.e. the complete phrase, context which decrypts the homonym, and assigns a unique value, creating the unambiguity of. Therefore mRNA as a kind of "phrase" or "offers" to work in protein synthesis as functional encodes an integer, Specifies the amino acid sequence of aminoacilirovannyh associates of tRNA, which complementary mRNA molecule to interact. The role and,R-parts of the Ribosome, If they are real, is akcepcii such associates — the predecessors of the protein with enzymatic by amino acids in peptide chain. In this case, there will be a context-oriented single-selection ex homonymous doublet-codons. You can predict the, the interaction of aminoacilirovannyh-tRNA to mRNA is collective phase character of type reassociacii ("annealing") odnotâžnyh DNA as the temperature drops after the "melting" of alternative polinukleotida.
Are there any experimental data, that could be interpreted in a manner? They are many and they are summarized in an analytical study [52]. Here are some of them.
Known, the correct recognition of tRNA molecules termination codons depends on their context environment, in particular, availability for stop codon uridine and, In addition, in the work of the[3] clearly shows:. Inserting a row of nine rarely used CUA-leucine codons after the 13th as part of the test mRNA codon 313 greatly inhibit their translation without an explicit impact on the translation of other mRNAs, CUA-containing codons. In Front Of The, a string of nine frequently-used CUG-lejcinovyh codons in the same positions did not have a pronounced effect on broadcast. While not rare, a frequently used codons do not affect this process, when they were introduced after the codon 223 or 307. Additional experiments have demonstrated, that strong positional effect rarely used codons can not be explained by differences in the stability of the mRNA or the severity of selecting the appropriate tRNA. The positional effect becomes clear, authors, assuming, that broadcast a sequence near the beginning of less stable reading: slow transmissions through a small use of codons, that used to follow in the message, and this leads to the disintegration of the broadcast products, sooner than will the full broadcast. As we can see, for the treatment of the own experiments involved cumbersome approval to split the broadcast products, assumptions, not following from their work, and which require specific and sensitive research. In this sense, our idea of contextual orientation in the management of the fusion proteins is simple, Although it is not easy to prove experimentally. Cited work well highlights the strategic line of strictly defined and distant from the place of education in kodonovyh the peptide mRNA on the inclusion or non-inclusion of particular amino acids in the protein synthesized. This is a distant influence, but in the work cited it simply stated, but for researchers not clear and, apparently, so not even discussed.
